|
|
|
|
| 下载文件名 |
Antibody Levels Poorly Reflect on the Frequency of Memory B Cells Generated following SARS-CoV-2, Seasonal Influenza, or EBV Infection |
发布时间 |
2023-03-18 |
下载次数 |
1 次 |
|
描述 |
The scope of immune monitoring is to define the existence, magnitude, and quality of
immune mechanisms operational in a host. In clinical trials and praxis, the assessment of humoral
immunity is commonly confined to measurements of serum antibody reactivity without accounting
for the memory B cell potential. Relying on fundamentally different mechanisms, however, passive
immunity conveyed by pre-existing antibodies needs to be distinguished from active B cell memory.
Here, we tested whether, in healthy human individuals, the antibody titers to SARS-CoV-2, seasonal
influenza, or Epstein–Barr virus antigens correlated with the frequency of recirculating memory B
cells reactive with the respective antigens. Weak correlations were found. The data suggest that the
assessment of humoral immunity by measurement of antibody levels does not reflect on memory
B cell frequencies and thus an individual’s potential to engage in an anamnestic antibody response
against the same or an antigenically related virus. Direct monitoring of the antigen-reactive memory
B cell compartment is both required and feasible towards that goal |
|
|
|
|
|
|
| 下载文件名 |
ELISPOT WORKSHOP |
发布时间 |
2015-01-04 |
下载次数 |
210 次 |
|
描述 |
Performance of four-color B cell and three-color T cell ELISPOT utilizing optimized, standardized protocols in a LIVE DEMONSTRATION — Detection of Immunoglobulin classes and IgG subclasses in a four-color ImmunoSpot ® Assay — Detection of IFN-γ, Granzyme B, and TNF-α in a three-color ImmunoSpot ® Assay Automated Live/Dead PBMC Counting using a CTL S6 Ultimate Fluorescent Analyzer — Assessing the quality of stored PBMC How Flow Cytometry and ELISPOT complement each other in immune diagnostics — Information that ELISPOT can provide but Flow Cytometry cannot Detecting B cell autoimmunity in Multiple Sclerosis — Diagnosis of the disease and the ability to monitor its activity Measuring B cell affinity ... And much, much more! |
|
|
|
|
|
|
| 下载文件名 |
PBMC Poster |
发布时间 |
2014-11-19 |
下载次数 |
23 次 |
|
描述 |
PBMC Poster |
|
|
|
|
|
|
| 下载文件名 |
Resting of Cryopreserved PBMC |
发布时间 |
2014-11-19 |
下载次数 |
23 次 |
|
描述 |
Resting of Cryopreserved PBMC Does Not
Generally Benefit the Performance of
Antigen-Specific T Cell ELISPOT Assays
(Publication)
|
|
|
|
